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Title: | Indonesian Pasuruan Propolis Extract Does Not Exert Antiproliferation and Pro-Apoptotic Effect on Human Colon Carcinoma Cell Line HT-29 |
Authors: | Kusuma, Ihda Dian Rosidah, Dian Norahmawati, Eviana Nurdiana, Nurdiana Endharti, Agustina Tri |
Keywords: | Bcl-xL Colon cancer Cyclin D1 Human colon adenocarcinoma cell line HT-29 Proliferation marker Ki67 Tumor protein p53 |
Issue Date: | 20-Jul-2018 |
Publisher: | AFRICAN JOURNALS ONLINE (AJOL) |
Series/Report no.: | Tropical Journal of Pharmaceutical Research; |
Abstract: | Purpose: To evaluate the anti-cancer activity of Indonesian Pasuruan propolis extract (PPE) against HT-29 human colon carcinoma cell line. Methods: HT-29 cells were cultured and treated in different concentrations of PPE (50, 100, 200, 400 μg/mL) for 24 h. The cells were evaluated by several indicators such as cell proliferation and apoptosis as well as the expression of protein Ki67, p53, cyclin D1, and Bcl-xL. Results: Administration of PPE inhibited cell proliferation of HT-29 in concentration-dependent manner but the effect was not significant (p = 0.842); the results were similar with regard to the expression of Ki67 in HT-29 cells (p = 0.953). Administration of 400 μg/mL PPE insignificantly decreased cyclin D1 expression (p = 0.149). The concentration of PPE at 50, 100, and 200 μg/mL induced cell apoptosis of HT-29 cells initially, but the level of apoptosis subsequently decreased (p = 0.416). Furthermore, the expressions of p53 and Bcl-xL decreased following treatment with PPE at 50, 100 and 200 μg/mL but increased for the PPE 400 μg/mL group (p = 0.000). Conclusion: PPE reduced the expressions of p53, Ki67, cyclin D1, and Bcl-xL insignificantly as it it generally failed inhibit cell proliferation and promote cell apoptosis of HT-29 cells. |
Description: | [ARCHIVES] Copyright Article from: Tropical Journal of Pharmaceutical Research (AFRICAN JOURNALS ONLINE (AJOL)) |
URI: | https://www.ajol.info/index.php/tjpr/article/view/175860 http://repository.unisma.ac.id/handle/123456789/2018 |
ISSN: | 1596-9827 |
Appears in Collections: | LP - Medical Education |
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dr.aris-1- 175860-Article Text-449822-1-10-20180807.pdf | Document | 536.17 kB | Adobe PDF | View/Open |
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