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http://repository.unisma.ac.id/handle/123456789/6203
Title: | Inhibitory activity of Urena lobata leaf extract on dipeptidyl peptidase-4 (DPP-4): Is it different in vitro and in vivo? |
Authors: | Purnomo, Yudi Soeatmadji, D.W Sumitro, S.B Widodo, Moch Aris |
Keywords: | Gly-pro-p-nitroanilide (GPPN) Urena lobata in vivo in vitro DPP-4 |
Issue Date: | 28-May-2018 |
Publisher: | Medicinal Plants - International Journal of Phytomedicines and Related Industries |
Series/Report no.: | International Journal of Phytomedicines and Related Industries;Year : 2018, Volume : 10, Issue : 2 |
Abstract: | This study was aimed to compare the anti diabetic effect of Urena lobata leaf extract through DPP-4 inhibitory activity by in vitro and in vivo. Urena lobata leaf was extracted in ethanol and hot water to evaluate its activity on DPP-4 both of in vitro and in vivo. In vitro test using Gly-pro-p-nitroanilide (GPPN) as substrate of DPP-4 and the reactions product of them was observed by microplate reader at λ=405 nm furthermore the IC50 value was determined. In vivo study utilize an animal model of diabetes with 2 control groups and 6 test groups (n=4), in which DPP-4 level, GLP-1 level and AUC of blood glucose were examined after extract administration. The in vitro DPP-4 inhibitory activity of ethanolic extract of U. lobata is higher than water extract with the IC50 value of 1654, 64 and 6489, 88 μg/ml respectively. However, the water extract of U. lobata exhibits stronger decrease DPP-4 level (60–70%) compared to ethanolic extract (40–60%) in vivo study as well as the AUC of blood glucose were reduced by 50–60% and 20–50%, respectively. Meanwhile, GLP-1 level could be retained more by the water extract of U. lobata administration (3–7 fold) compared to ethanolic extract (25 fold) due to the reducing of DPP-4 activity. |
Description: | [ARCHIVES] Copyright Article From: International Journal of Phytomedicines and Related Industries |
URI: | https://www.indianjournals.com/ijor.aspx?target=ijor:mpijpri&volume=10&issue=2&article=002 http://repository.unisma.ac.id/handle/123456789/6203 |
ISSN: | 0975-6892 |
Appears in Collections: | LP - Pharmaceutical |
Files in This Item:
File | Description | Size | Format | |
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Corr-MedicinalPlant-IJPRI_Inhibit.pdf | 1.97 MB | Adobe PDF | View/Open | |
4 IJPRI_ALL.pdf | 2.49 MB | Adobe PDF | View/Open |
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