Publikasi Ilmiah Dosen Program Studi Pendidikan Dokter https://repository.unisma.ac.id/handle/123456789/99 2026-07-14T11:31:22Z 2026-07-14T11:31:22Z Widiyana, Anita Puspa https://repository.unisma.ac.id/handle/123456789/7184 2023-05-22T02:53:46Z 2022-12-10T00:00:00Z

HUBUNGAN KUANTITATIF STRUKTUR-PROPERTI (HKSP) SENYAWA AKTIF TEMU KUNCI (BOESENBERGIA PANDURATA) SEBAGAI PENGHAMBAT ENZIM MAIN PROTEASE (MPRO) SARS-CoV-2 Widiyana, Anita Puspa Hubungan parameter fisikokimia dari 15 senyawa aktif Boesenbergia pandurata dalam menghambat enzim main protease SARS-CoV-2 diprediksi melalui model Hubungan Kuantitatif Struktur-Properti (HKSP). Semua senyawa aktif diperoleh dari basis data. Parameter fisikokimia meliputi sifat lipofilik, elektronik dan sterik ditentukan dengan ChemDraw sedangkan prediksi aktivitas diambil dari penelitian sebelumnya. Hubungan kuantitatif dari parameter fisikokimia dan aktivitas dianalisis menggunakan regresi linier berganda dengan SPSS. Persamaan terbaik dipilih berdasarkan nilai r, R2, SE, F, dan .sig. Persamaan yang diperoleh sebanyak 41 persamaan dan persamaan terbaiknya yaitu ΔG= (-0,476*log P) - (0,022*ELUMO) - (0,019*BM) - 1,121 (n= 15; r= 0,972; R2= 0,946; SE = 0,281; F= 63,712; .sig = 0,000). Parameter yang diprediksi paling berpengaruh secara simultan dan signifikan terhadap aktivitas dalam menghambat enzim main protease SARS-CoV-2 adalah Log P, ELUMO dan berat molekul.

2022-12-10T00:00:00Z Yang, Dongqing Anderson, Paul H. Wijeyanaka, Asiri R. Barratt, Kate R. Triliana, Rahma Stapledon, Catherine J.M. Zhou, Hong Findlay, David M. Morris, Howard A. Atkins, Gerald J. https://repository.unisma.ac.id/handle/123456789/3111 2022-02-23T05:13:43Z 2018-01-01T00:00:00Z

Both ligand and VDR expression levels critically determine the effect of 1α,25-dihydroxyvitamin-D3 on osteoblast differentiation Yang, Dongqing; Anderson, Paul H.; Wijeyanaka, Asiri R.; Barratt, Kate R.; Triliana, Rahma; Stapledon, Catherine J.M.; Zhou, Hong; Findlay, David M.; Morris, Howard A.; Atkins, Gerald J. Previous studies have shown that 1α,25-dihydroxyvitamin D3 (1,25D) through vitamin D receptor (VDR) signalling has both catabolic and anabolic effects on osteoblast differentiation. However, the mechanism of these differential effects by 1,25D is not fully understood. In this study, mice with three different genetic backgrounds, representing a normal VDR level (wild-type, WT), VDR over-expression specifically in mature osteoblasts (ObVDR-B6) and global VDR knockout (VDRKO), were utilised to generate primary osteoblast-like cultures to further elucidate the effects of 1,25D on osteoblast differentiation. Our data confirm the importance of VDR in the late stage of osteogenic differentiation and also for the expression of factors critical for osteoblastic support of osteoclast formation. This study also demonstrates the differential effects of a pharmacological level of 1,25D (1 nM) on the expression of osteogenic differentiation markers, including Ocn and Sost, depending on the relative level of VDR. Our findings suggest that 1,25D plays an inhibitory role in matrix mineralisation, possibly through the modulation of the tissue non-specific alkaline phosphatase to ectonucleotide pyrophosphatase/phosphodiesterase 1 axis, in a VDR level-dependent manner. We conclude that the relative VDR level and the 1,25D availability to cells, are important co-determinants for whether 1,25D plays a promoting or suppressive role in osteoblast-mediated osteogenic activity. [ARCHIVES] Copyright Article from: The Journal of Steroid Biochemistry and Molecular Biology

2018-01-01T00:00:00Z Yeh, Jwu-Lai Sulistyowati, Erna https://repository.unisma.ac.id/handle/123456789/3110 2022-02-23T05:12:39Z 2018-01-01T00:00:00Z

Cardiovascular protective effect of Indonesian herbal medicine in spontaneously hypertensive rats Yeh, Jwu-Lai; Sulistyowati, Erna [ARCHIVES] Copyright Article from: J-STAGE

2018-01-01T00:00:00Z Huang, Shang-En Sulistyowati, Erna Chao, Yu-Ying Wu, Bin-Nan Dai, Zen-Kong Hsu, Jong-Hau Yeh, Jwu-Lai https://repository.unisma.ac.id/handle/123456789/2578 2021-11-18T02:52:24Z 2021-05-28T00:00:00Z

In Vitro Evaluation of the Anti-Inflammatory Effect of KMUP-1 and In Vivo Analysis of Its Therapeutic Potential in Osteoarthritis Huang, Shang-En; Sulistyowati, Erna; Chao, Yu-Ying; Wu, Bin-Nan; Dai, Zen-Kong; Hsu, Jong-Hau; Yeh, Jwu-Lai Osteoarthritis is a degenerative arthropathy that is mainly characterized by dysregulation of inflammatory responses. KMUP-1, a derived chemical synthetic of xanthine, has been shown to have anti-inflammatory and antioxidant properties. Here, we aimed to investigate the in vitro anti-inflammatory and in vivo anti-osteoarthritis effects of KMUP-1. Protein and gene expressions of inflammation markers were determined by ELISA, Western blotting and microarray, respectively. RAW264.7 mouse macrophages were cultured and pretreated with KMUP-1 (1, 5, 10 µM). The productions of TNF-α, IL-6, MMP-2 and MMP- 9 were reduced by KMUP-1 pretreatment in LPS induced inflammation of RAW264.7 cells. The expressions of iNOS, TNF-α, COX-2, MMP-2 and MMP-9 were also inhibited by KMUP-1 pretreatment. The gene expression levels of TNF and COX families were also downregulated. In addition, KMUP-1 suppressed the activations of ERK, JNK and p38 as well as phosphorylation of IκBα/NF-κB signaling pathways. Furthermore, SIRT1 inhibitor attenuated the inhibitory effect of KMUP-1 in LPS-induced NF-κB activation. In vivo study showed that KMUP-1 reduced mechanical hyperalgesia in monoiodoacetic acid (MIA)-induced rats OA. Additionally, KMUP-1 pretreatment reduced the serum levels of TNF-α and IL-6 in MIA-injected rats. Moreover, macroscopic and histological observation showed that KMUP-1 reduced articular cartilage erosion in rats. Our results demonstrated that KMUP-1 inhibited the inflammatory responses and restored SIRT1 in vitro, alleviated joint-related pain and cartilage destruction in vivo. Taken together, KMUP-1 has the potential to improve MIA-induced articular cartilage degradation by inhibiting the levels and expression of inflammatory mediators suggesting that KMUP-1 might be a potential therapeutic agent for OA. [ARCHIVES] Copyright Article from : Biomedicines 2021

2021-05-28T00:00:00Z