LPP - Animal Husbandry

The Comparison of Chromosome Analysis Result by Manual and Software Cytovision Image Analysis Using Simple G-Banding

2017-08-01T00:00:00Z

The Comparison of Chromosome Analysis Result by Manual and Software Cytovision Image Analysis Using Simple G-Banding Ciptadi, Gatot; Ihsan, Muhammad Nur; Nurgiartiningsih, Veronica Margaretha; Mudawamah; Putri, Ardyah Ramadhina Irsanti Chromosome analysis or karyotyping is one among powerful methods to characterize normal or abnormal genetic of animals. On the basis of the important chromosome abnormalities and their negative effect in the near future, chromosomal investigation of breeding bull especially for Artificial Insemination (AI) began in different countries. Chromosomal abnormalities are usually considered to be a plague and are to eliminate. In Indonesia, AI implementation in cattle have been started intensively, especially using imported bulls (bos Taurus) i.e. Limousine. A number of cattle breeds have been reported on of the 50 exotic breeds with the problem of 1/29 translocation, then the chromosome analysis to be important to execute. Method performed by collecting blood samples from first generation of crossing breed Madura Cattle vs. Bos Taurus. Sample was added to medium (Karyo MAX Gibco) then placed in CO2 incubator at 38o C. Colcichine was added aafter 70 hours and kept for 2-3 hours. Slides were prepared and dried then stained with Giemsa. Slides were examined under high phase-contrast microscope, chromosome analysis using cytovision software and manual analysis straightly captured under microscope then arranged. Result of both method of kariotyping may accepted for analysing method of abnormal/normal chromosome. It showed that the 2 N diploid number of chromosome was normal was 60, there were 58 autosome and 2 sex chromosome in all cattle observed. It was observed that all cattle tested in this research were normal categories. The karyotype analysis of all cattle showed that the chromosomes of one cell and different individual each breed varied in size, shape and position of centromere. It was recommended to performed chromosomal investigation of breeding bulls using advanced sophisticated tools of analysis like cytovision image analysis of fluorescent technique. Manual method was recommended only for analysis of normal or abnormal number of chromosome. [ARCHIVES] Copyright Article from : RESEARCH JOURNAL of LIFE SCIENCE

Variasi Fenotipe Morfometri Burung Kenari Dewasa antara Warna Bulu Terang Kuning dan Putih

2013-01-01T00:00:00Z

Variasi Fenotipe Morfometri Burung Kenari Dewasa antara Warna Bulu Terang Kuning dan Putih M., Auzaini; Mudawamah; Suryanto, D.; Fadli, M.Z. Burung kenari merupakan salah satu jenis burung berkicau yang banyak digemari masyarakat Indonesia. Burung kenari yang menjadi favorit peternak diantarnya adalah burung kenari warna bulu kuning dan putih. Tujuan penelitian untuk melihat perbedaan ukuran tubuh burung kenari dewasa dengan warna bulu terang kuning dan putih. Metode penelitian yang digunakan adalah metode survei di lapangan dengan pengambilan sampel secara purposive sampling yaitu sampel kenari yang sudah jadi induk dan pejantan dengan warna bulu kuning dan putih. Variabel yang diamati meliputi panjang femur, panjang tibia, panjang shank, panjang jari, panjang mandibula, panjang tubuh, panjang tulang sayap, panjang tulang dada, panjang lingkar shank. Hasil penelitian menujukkan bahwa ukuran tubuh pada burung kenari kuning jantan berbeda nyata (P < 0,05) lebih panjang dibandingkan dengan kenari putih jantan dan burung kenari kuning betina dibandingkan dengan burung kenari putih betina. Sebaliknya ukuran tubuh tidak berbeda nyata (P > 0,05) antara burung kenari kuning jantan dan burung kenari putih jantan dan antara burung kenari warna bulu kuning betina dengan burung kenari warna bulu putih betina. Kesimpulan hasil penelitian ini adalah rataan morfometri burung kenari warna bulu kuning lebih panjang dibandingkan dengan burung kenari warna bulu putih; Variasi fenotipe morfometri (panjang tibia, jari, tubuh, sayap, dan dada) pada burung kenari jantan warna bulu kuning polos nyata lebih panjang dibandingkan dengan warna bulu putih polos. Variasi fenotipe morfometri (panjang femur, tibia, shank, jari, mandibula dan dada) pada kenari betina warna bulu kuning polos nyata lebih panjang dibandingkan dengan warna bulu putih polos. [ARCHIVES] Copyright Article from : TERNAK TROPIKA : Journal of Tropical Animal Production (JTAPRO)

Chemical Composition of Vermicompost Made from Organic Wastes through the Vermicomposting and Composting with the Addition of Fish Meal and Egg Shells Flour

2017-05-05T00:00:00Z

Chemical Composition of Vermicompost Made from Organic Wastes through the Vermicomposting and Composting with the Addition of Fish Meal and Egg Shells Flour Nurhidayati, N.; Usman, Ali; Muwarni, Indiyah Chemical composition of compost is an important indicator that determines the quality of compost. This study compared the chemical composition of vermicompost resulting from the process of vermicomposting alone with combined vermicomposting and composting with addition of egg shells flour and fish meal. Organic wastes used were the mixture of spent mushrooms waste, coconut husks, cow dung, vegetables residue, and leaf litter. Lumbricus rubellus was the species of earthworm used in the vermicomposting process. In the composting process, egg shells flour and fish meal are added into the vermicompost as additives materials. The results indicate that the combined vermicomposting and composting process with addition the additives materials improves the chemical composition of vermicompost compared to using vermicomposting process alone. The change of chemical composition was indicated by a decrease in C-organic content and C/N ratio by 29% and 99%, respectively, while the content of N, P, K and S increased by 52%, 67.5%, 29% and 25%, respectively due to the addition of additives material in the composting process. The largest increase of vermicompost nutrient content occurred in the Ca content by an average of up to 7-fold. While polyphenols, lignin and cellulose content of vermicompost decreased slightly. The treatment of two mixture (a) spent mushrooms waste, cow dung and vegetables residue, and (b) spent mushroom waste, cow dung, vegetables residue, and leaf litter gave the best chemical composition. However, to determine the quality, we need to test the product in a plant growth bioassay as a follow-up study. [ARCHIVES] Copyright Article from : The Journal of Pure and Applied Chemistry Research