Subsitusi Fitohormon Dengan Air Kelapa (Cocos nucifera L.) Pada Medium Vacin and Went terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp Secara In Vitro

Abstract

Research on Phytohormone Substitution with Coconut Water (Cocos nucifera L.) on VW Medium on the Growth of Dendrobium sp. Orchids In vitro, was conducted at the DD Orchid Nursery Tissue Culture Laboratory, Dadaprejo, Junrejo, Kab. Batu, East Java. Starting from November to January 2020. The purpose of this study was to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study uses an experimental method, descriptive data analysis to compare several different concentrations of Coconut Water. The design of this study used a Completely Randomized Design (CRD) consisting of 1 factor, namely Coconut Water using 4 levels of Coconut Water concentration, namely 0% (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle carried out for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and 10.8 leaves. The highest number of roots and the longest root length were produced at 600 ml / L coconut water concentration (60% concentration) with an average of 6 roots, and the longest average length of 0.5 cm. Keywords: Young coconut water (Cocos nucifera L.), Dendrobium sp., In vitro, growth.