Treatment for endoparasitic infection or intestinal helmints in dairy cows (Bos taurus) usually uses chemical drugs, but continuous administration can develop of drug resistance. One effort that can be done is to replace chemicals with natural ingredients in the form of papaya leaf (Carica papaya). Papaya leaf contain alkaloids, enzyme papain, saponin, flavonoid, and tannin. The purpose of this experiment was to analyze the effect of papaya leaf (Carica papaya) on endoparasites and milk harvesting. This is an experimental research used a pretest post-test control group design on five dairy cows with one treatment, specifically 70% basal green feed and 30% papaya leaves and one positive control (Albendazole). Method of identification use the qualitative method is, native method (direct slide) and the flotation method (Flotation method). The obtained data were analyzed with a Anova test, it showed that result is (p<0,05). That are able to suppress the growth of endoparasites in cow 1, 2, 3, 4, 5 by 60%, 58%, 50%, 45% , and 34% respectively in the native method and the floating method are 75%, 63%, 56%, 45%, and 34%. Increased harvesting milk for 3 weeks is 2-4 liters. It was conluded that there was an influence between papaya leaves (Carica papaya) on endoparasites in dairy cows (Bos taurus) as a natural antihelmintic. Key words: Antihelmintic, Carica papay), Papaya Leaf, Endoparasite, Cows (Bos taurus), Milk Harvesting Result