Abstract:
Chromosome analysis or karyotyping is
one among powerful methods to characterize
normal or abnormal genetic of animals. On the
basis of the important chromosome
abnormalities and their negative effect in the
near future, chromosomal investigation of
breeding bull especially for Artificial
Insemination (AI) began in different countries.
Chromosomal abnormalities are usually
considered to be a plague and are to
eliminate. In Indonesia, AI implementation in
cattle have been started intensively, especially
using imported bulls (bos Taurus) i.e.
Limousine. A number of cattle breeds have
been reported on of the 50 exotic breeds with
the problem of 1/29 translocation, then the
chromosome analysis to be important to
execute. Method performed by collecting
blood samples from first generation of
crossing breed Madura Cattle vs. Bos Taurus.
Sample was added to medium (Karyo MAX
Gibco) then placed in CO2 incubator at 38o C.
Colcichine was added aafter 70 hours and kept
for 2-3 hours. Slides were prepared and dried
then stained with Giemsa. Slides were
examined under high phase-contrast
microscope, chromosome analysis using
cytovision software and manual analysis
straightly captured under microscope then
arranged. Result of both method of
kariotyping may accepted for analysing
method of abnormal/normal chromosome. It
showed that the 2 N diploid number of
chromosome was normal was 60, there were
58 autosome and 2 sex chromosome in all
cattle observed. It was observed that all cattle
tested in this research were normal
categories. The karyotype analysis of all cattle
showed that the chromosomes of one cell and
different individual each breed varied in size,
shape and position of centromere. It was
recommended to performed chromosomal
investigation of breeding bulls using advanced
sophisticated tools of analysis like cytovision
image analysis of fluorescent technique.
Manual method was recommended only for
analysis of normal or abnormal number of
chromosome.
