Individual Mutations in Indonesian Local Ettawah Goats Based on The GDF9 Gene

Abstract

A prolific trait was essential to determine the litter size in goats including Indonesian Local Ettawah Goats (ILEG), and one of the genes that influence the prolific trait was the GDF9 gene. The purpose of this study was to describe the amino acid expression of the GDF9 gene sequencing in ILEG compared with Genbank accession number GU784823.2. Capra hircus GDF9. This research method was an experiment which includes the sampling of 21 does who had given birth more than once with low, medium and high prolific categories. Furthermore, samples were PCR with exon 1 GDF9 gene, and sequencing results were carried out by amino acid alignment with BioEdit software. The results showed that there were 3 variants in the exon 1 GDF9 gene in ILEG which was located at the location of 27, 61 and 85 amino acid residues. At the 27th residue there was a change in the proline amino acid to alanine (CCT to GCT), at the 61st residue there was no change in the amino acid Leucine, but there was a change in the base N compound (CTA to CTC), and at 85 residues there was a change in acid residue amino Alanine becomes Glycine (GCT to GGT).