The semen’s storage process required thinners containing food substances and must be able to protect spermatozoa during the storage process. One of the most common problems in semen’s dilution is a decrease in spermatozoa’s motility. The study aims to analyze the effect of tris egg yolk and andromed diluent against the FH (Friesian Holstein) cattle’s cement spermatozoa motility before and after the freezing. This research uses the experimental method with a randomized block design. It consist of 2 treatments, that are P1 = FH cattle’s semen that is thinned using an tris egg yolk and P2 = FH cattle’s semen that is thinned an andromed diluent. There are 4 sample group of semen from 4 cattle and it acted out 4 times. The parameter used for measurement in this research is the motility of the spermatozoa. The obtained data were analyzed using ANOVA test and post hoc test. From ANOVA test, it showed that there are a real distinction (P<0,01) between tris egg yolk and andromed diluent in the reaction of FH cattle’s semen spermatozoa’s motility value. The percentage of sperm motility of semen before coagulation process is 55% for each group, however tris egg yolk thinners are more able to maintain the motility or impulses of semen spermatozoa of the Holstein Friesian cows compared to andromed thinners after freezing. The value of frozen semen spermatozoa’s motility with tris egg yolk are 4,1±2,50 for first and fourth group and 42,5±28,9 for the second and the third group. While with andromed diluent result 26,3±7,50 for the first group, 25,0±5,77 for the second group, 17,5±5,00 for the third group, and 18,8±77,50 for the fourth group. Keywords : andromed, Bos taurus, spermatozoa’s motility, tris egg yolk.