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Title: | Anti-diabetic potential of Urena lobata leaf extract through inhibition of dipeptidyl peptidase IV activity |
Authors: | Purnomo, Yudi Soeatmadji, D.W Sumitro, S.B Widodo, M.A |
Keywords: | Anti-diabetic Dipeptidyl peptidase IV In silico In vitro Urena lobata |
Issue Date: | 1-Aug-2015 |
Publisher: | Asian Pacific Journal of Tropical Biomedicine |
Citation: | Yudi Purnomo, Djoko Wahono Soeatmadji, Sutiman Bambang Sumitro, Mochamad Aris Widodo, Anti-diabetic potential of Urena lobata leaf extract through inhibition of dipeptidyl peptidase IV activity, Asian Pacific Journal of Tropical Biomedicine, Volume 5, Issue 8, 2015 |
Series/Report no.: | Asian Pacific Journal of Tropical Biomedicine;Volume 5, Issue 8 |
Abstract: | Objective To evaluate the anti-diabetic potential of leaf extract from Urena lobata (U. lobata) through dipeptidyl peptidase IV (DPP-IV) inhibitory activity. Methods U. lobata leaf was extracted in hot water and ethanol. The activity of DPP-IV inhibitor was tested by in vitro study using gly-pro-p-nitroanilide as substrat of DPP-IV and vildagliptin, as standard reference. A product of the reactions between gly-pro-p-nitroanilide and DPP-IV, was observed by microplate readers with λ = 405 nm. All data were expressed as mean ± SD and the IC50 value was determined by non linear regression curve fit. Active substances in leaf extract of U. lobata was analyzed by liquid chromatography-mass spectrometry. DPP-IV inhibitory activity of active compounds was evaluated in silico using docking server. Results The ethanolic extract of U. lobata showed stronger DPP-IV inhibitor activity than water extract with the IC50 values of 1 654.64 and 6 489.88 μg/mL, respectively. Vildagliptin, based on standard reference for DPP-IV inhibitor activity, has IC50 value of 57.44 μg/mL. Based on in silico analysis, mangiferin, stigmasterol and β-sitosterol in U. lobata extract have a strong inhibitory activity on DPP-IV. Conclusions The results showed that DPP-IV inhibitory activity of U. lobata is related to its active compounds such as mangiferin, stigmasterol and β-sitosterol. |
Description: | [ARCHIVES] Copyright Article From: Asian Pacific Journal of Tropical Biomedicine |
URI: | https://www.sciencedirect.com/science/article/pii/S2221169115001124 http://repository.unisma.ac.id/handle/123456789/6325 |
ISSN: | 2221-1691 |
Appears in Collections: | LP - Pharmaceutical |
Files in This Item:
File | Description | Size | Format | |
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Corr-APJTB_anti-diabetic.pdf | Content | 2.63 MB | Adobe PDF | View/Open |
1 APJTB_ALL.pdf | 2.61 MB | Adobe PDF | View/Open |
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