The difference of map1lc3 level as macrophage autophagy Marker between resistant and sensitive tuberculosis patients on rifampicin

Wulandari, Dian N; Nugraha, Jusak; Soedarsono, Soedarsono

dc.contributor.author	Wulandari, Dian N
dc.contributor.author	Nugraha, Jusak
dc.contributor.author	Soedarsono, Soedarsono
dc.date.accessioned	2021-10-11T06:27:48Z
dc.date.available	2021-10-11T06:27:48Z
dc.date.issued	2018-04-01
dc.identifier.issn	2356-0991
dc.identifier.uri	https://e-journal.unair.ac.id/IJTID/article/view/6024
dc.identifier.uri	http://repository.unisma.ac.id/handle/123456789/1922
dc.description	[ARCHIVES] Copyright Article from: Indonesian Journal of Tropical and Infectious Disease	en_US
dc.description.abstract	M. tuberculosis (MTB) is an intracelular bacteria that live in the host macrophage cells. Several organs can be affected by tuberculosis but most major illnesses are lung diseases. Immediately after infection, MTB will be phagocytosed by the alveolar macrophage cells and can survive in the phagosome. The macrophage plays a role in innate immunity towards an infection using autophagy by removing the microbe directly via phagocytosis. When bacteria phagocytosized, vacuole membrane formed double membranes called autophagosome, and followed by degradation by lysosome, which known as autolysosome. Induction of autophagy can be observed on the formation of microtubule-associated proteins 1B lightchain 3B (MAP1LC3B/LC3). MAP1LC3B is protein that have role at autophagic way for selection autophagy substrate and biogenesis. In this study we are used serum from patients TB with rifampicin resistant and rifampicin sensitive as control. Samples were divided using gene expert to differentiate between resistant and sensitive rifampicin.This research aims to compare MAP1LC3B levels in resistant and sensitive rifampicin to study macrophages respond in autophagic way in tuberculosis patients, and give information for define therapy plan to improve therapy for MDR-TB patients. Type of this research is a case control study design with cross sectional research with each groups sample is 19 from age 18-65 years old. Result, MAP1LC3B serum levels on the rifampicin resistant group are lower compared to rifampicin sensitive group. This occur because MTB is able to hide and evade innate immune defense mechanisms. MTB can maintain intracellular growth inside the phagosome by inhibiting phagolysosome formation in autophagy process especially inhibit MAP1LC3B formation by PDIM.	en_US
dc.language.iso	en	en_US
dc.publisher	Lembaga Penyakit Tropis (Institute of Tropical Disease) Universitas Airlangga	en_US
dc.relation.ispartofseries	Indonesian Journal of Tropical and Infectious Disease;Vol.7, Issue 1, Page 6-10
dc.subject	mycobacterium tuberculosis	en_US
dc.subject	drug resistance	en_US
dc.subject	rifampicin	en_US
dc.subject	autophagy	en_US
dc.subject	MAP1LC3B	en_US
dc.title	The difference of map1lc3 level as macrophage autophagy Marker between resistant and sensitive tuberculosis patients on rifampicin	en_US
dc.type	Article	en_US