Inhibitory activity of Urena lobata leaf extract on dipeptidyl peptidase-4 (DPP-4):

Yudi, Purnomo; Djoko, Soeatmadji; Bambang, Sumitro Sutiman; Aris, Widodo Moch

dc.contributor.author	Yudi, Purnomo
dc.contributor.author	Djoko, Soeatmadji
dc.contributor.author	Bambang, Sumitro Sutiman
dc.contributor.author	Aris, Widodo Moch
dc.date.accessioned	2021-10-11T06:42:23Z
dc.date.available	2021-10-11T06:42:23Z
dc.date.issued	2018-05-25
dc.identifier.issn	0975-6892
dc.identifier.uri	http://www.indianjournals.com/ijor.aspx?target=ijor:mpijpri&volume=10&issue=2&article=002
dc.identifier.uri	http://repository.unisma.ac.id/handle/123456789/1927
dc.description	[ARCHIVES] Copyright Article from: International Journal of Phytomedicines and Related Industries	en_US
dc.description.abstract	This study was aimed to compare the anti diabetic effect of Urena lobata leaf extract through DPP-4 inhibitory activity by in vitro and in vivo. Urena lobata leaf was extracted in ethanol and hot water to evaluate its activity on DPP-4 both of in vitro and in vivo. In vitro test using Gly-pro-p-nitroanilide (GPPN) as substrate of DPP-4 and the reactions product of them was observed by microplate reader at λ=405 nm furthermore the IC50 value was determined. In vivo study utilize an animal model of diabetes with 2 control groups and 6 test groups (n=4), in which DPP-4 level, GLP-1 level and AUC of blood glucose were examined after extract administration. The in vitro DPP-4 inhibitory activity of ethanolic extract of U. lobata is higher than water extract with the IC50 value of 1654, 64 and 6489, 88 μg/ml respectively. However, the water extract of U. lobata exhibits stronger decrease DPP-4 level (60–70%) compared to ethanolic extract (40–60%) in vivo study as well as the AUC of blood glucose were reduced by 50–60% and 20–50%, respectively. Meanwhile, GLP-1 level could be retained more by the water extract of U. lobata administration (3–7 fold) compared to ethanolic extract (25 fold) due to the reducing of DPP-4 activity.	en_US
dc.language.iso	en	en_US
dc.publisher	Society For Conservation And Resource Development Of Medicinal Plants	en_US
dc.relation.ispartofseries	International Journal of Phytomedicines and Related Industries;Vol.10, Issue 2, Pages 99-105
dc.subject	DPP-4	en_US
dc.subject	in vitro	en_US
dc.subject	in vivo	en_US
dc.subject	Urena lobata	en_US
dc.subject	Gly-pro-p-nitroanilide (GPPN)	en_US
dc.title	Inhibitory activity of Urena lobata leaf extract on dipeptidyl peptidase-4 (DPP-4):	en_US
dc.title.alternative	Is it different in vitro and in vivo?	en_US
dc.type	Article	en_US