Abstract:
Purpose of this research was to investigate the effect of Bovine Serum
Albumin substitution by albumen on CEP-2 to semen quality Ongole CrossBred bull
stored at 3-5
oC. Research was conducted at Research Centre Beef Cattle Laboratory,
Grati, Pasuruan on February 2016. Semen diluent was divided into two groups, there
were P0 (90% CEP-2 + 10% Egg Yolk (EY)); and P1 (90% CEP + 0.4% albumen +
10% EY). Data of the research were analyzed using paired design t test. The result
showed that after eight days chilled preservation, percentage of motility P1
(47.4±10.9%) was higher than P0 (47±9.2%). Percentage of viability P0 (83.1±1.9%)
was higher than P1 (81.3±1.5%). Percentage of abnormality P1 (3.6±0.4%) was lower
than P0 (3.8±0.3%). Total motile sperm count after six days chilled preservation was
significantly higher in all treatments compared to the standard criteria of SNI 40%
motile sperm/ml. The conclusion of this research was 0,4% albumen could replace the
BSA capability on CEP-2.
