Indonesian Pasuruan Propolis Extract Does Not Exert Antiproliferation and Pro-Apoptotic Effect on Human Colon Carcinoma Cell Line HT-29

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dc.contributor.author Kusuma, Ihda Dian
dc.contributor.author Rosidah, Dian
dc.contributor.author Norahmawati, Eviana
dc.contributor.author Nurdiana, Nurdiana
dc.contributor.author Endharti, Agustina Tri
dc.date.accessioned 2021-10-13T03:53:48Z
dc.date.available 2021-10-13T03:53:48Z
dc.date.issued 2018-07-20
dc.identifier.issn 1596-9827
dc.identifier.uri https://www.ajol.info/index.php/tjpr/article/view/175860
dc.identifier.uri http://repository.unisma.ac.id/handle/123456789/2018
dc.description [ARCHIVES] Copyright Article from: Tropical Journal of Pharmaceutical Research (AFRICAN JOURNALS ONLINE (AJOL)) en_US
dc.description.abstract Purpose: To evaluate the anti-cancer activity of Indonesian Pasuruan propolis extract (PPE) against HT-29 human colon carcinoma cell line. Methods: HT-29 cells were cultured and treated in different concentrations of PPE (50, 100, 200, 400 μg/mL) for 24 h. The cells were evaluated by several indicators such as cell proliferation and apoptosis as well as the expression of protein Ki67, p53, cyclin D1, and Bcl-xL. Results: Administration of PPE inhibited cell proliferation of HT-29 in concentration-dependent manner but the effect was not significant (p = 0.842); the results were similar with regard to the expression of Ki67 in HT-29 cells (p = 0.953). Administration of 400 μg/mL PPE insignificantly decreased cyclin D1 expression (p = 0.149). The concentration of PPE at 50, 100, and 200 μg/mL induced cell apoptosis of HT-29 cells initially, but the level of apoptosis subsequently decreased (p = 0.416). Furthermore, the expressions of p53 and Bcl-xL decreased following treatment with PPE at 50, 100 and 200 μg/mL but increased for the PPE 400 μg/mL group (p = 0.000). Conclusion: PPE reduced the expressions of p53, Ki67, cyclin D1, and Bcl-xL insignificantly as it it generally failed inhibit cell proliferation and promote cell apoptosis of HT-29 cells. en_US
dc.language.iso en en_US
dc.publisher AFRICAN JOURNALS ONLINE (AJOL) en_US
dc.relation.ispartofseries Tropical Journal of Pharmaceutical Research;
dc.subject Bcl-xL en_US
dc.subject Colon cancer en_US
dc.subject Cyclin D1 en_US
dc.subject Human colon adenocarcinoma cell line HT-29 en_US
dc.subject Proliferation marker Ki67 en_US
dc.subject Tumor protein p53 en_US
dc.title Indonesian Pasuruan Propolis Extract Does Not Exert Antiproliferation and Pro-Apoptotic Effect on Human Colon Carcinoma Cell Line HT-29 en_US
dc.type Article en_US


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