Comparison of DNA Extraction Methods Between Conventional, Kit, Alkali and Buffer-Only for PCR Amplification on Raw and Boiled Bovine and Porcine Meat

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dc.contributor.author Yahya, Arif
dc.contributor.author Firmansyah, Marindra
dc.contributor.author Arlisyah, Annisa
dc.contributor.author Risandiansyah, Rio
dc.date.accessioned 2021-10-14T01:48:43Z
dc.date.available 2021-10-14T01:48:43Z
dc.date.issued 2017
dc.identifier.issn 2087-2852
dc.identifier.uri https://jels.ub.ac.id/index.php/jels/article/view/243
dc.identifier.uri http://repository.unisma.ac.id/handle/123456789/2035
dc.description [ARCHIVES] Copyright Article from: The Journal of Experimental Life Science (Universitas Brawijaya) en_US
dc.description.abstract Detection of porcine contamination in food material by employing PCR techniques is integral in halal food confirmation. However, PCR is both costly and laborious, particularly in DNA isolation method. This study explores several different methods in DNA extraction for PCR amplification in bovine and porcine raw and boiled meat samples. Four methods for DNA extraction (conventional PCI method, DNA isolation kit, alkaline-based method, and a DNA lysis buffer-only from the same kit) was employed followed by PCR using primers from previous studies and compared for DNA quality and quantity (in six replicates) and PCR amplification on the best three DNA samples. This study shows that in all samples, the conventional method had the best DNA yield based on nanodrop measurement, followed by an alkali-based method, buffer-only method, and DNA isolation kit. Each method except lysis-buffer only had at least one sample with good DNA quality. Conventional and isolation kit showed reliable positive PCR detection for all porcine and bovine samples (92% positive). Using the alkaline-lysis method, DNA was amplified reliably on boiled meat samples (83% positive). Lysis-buffer-only method did not show consistent PCR amplification on the samples used (50% positive). The conclusion was that conventional PCI method and DNA isolation kit showed high reliability in PCR amplification of bovine and porcine meats, both raw and boiled. While high DNA yield was obtained using the alkaline-lysis method, PCR amplification was only successful on boiled samples. Lysis-buffer only method yielded in poor DNA quality and was not able to result in reliable DNA amplification en_US
dc.language.iso en en_US
dc.publisher Universitas Brawijaya en_US
dc.relation.ispartofseries The Journal of Experimental Life Science;Vol.7, Issue 2, Page 110-114
dc.subject DNA extraction methods en_US
dc.subject Halal en_US
dc.subject Porcine food contaminants. en_US
dc.title Comparison of DNA Extraction Methods Between Conventional, Kit, Alkali and Buffer-Only for PCR Amplification on Raw and Boiled Bovine and Porcine Meat en_US
dc.type Article en_US


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