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dc.contributor.authorYahya, Arif
dc.contributor.authorFirmansyah, Marindra
dc.contributor.authorArlisyah, Annisa
dc.contributor.authorRisandiansyah, Rio
dc.date.accessioned2021-10-14T01:48:43Z
dc.date.available2021-10-14T01:48:43Z
dc.date.issued2017
dc.identifier.issn2087-2852
dc.identifier.urihttps://jels.ub.ac.id/index.php/jels/article/view/243
dc.identifier.urihttp://repository.unisma.ac.id/handle/123456789/2035
dc.description[ARCHIVES] Copyright Article from: The Journal of Experimental Life Science (Universitas Brawijaya)en_US
dc.description.abstractDetection of porcine contamination in food material by employing PCR techniques is integral in halal food confirmation. However, PCR is both costly and laborious, particularly in DNA isolation method. This study explores several different methods in DNA extraction for PCR amplification in bovine and porcine raw and boiled meat samples. Four methods for DNA extraction (conventional PCI method, DNA isolation kit, alkaline-based method, and a DNA lysis buffer-only from the same kit) was employed followed by PCR using primers from previous studies and compared for DNA quality and quantity (in six replicates) and PCR amplification on the best three DNA samples. This study shows that in all samples, the conventional method had the best DNA yield based on nanodrop measurement, followed by an alkali-based method, buffer-only method, and DNA isolation kit. Each method except lysis-buffer only had at least one sample with good DNA quality. Conventional and isolation kit showed reliable positive PCR detection for all porcine and bovine samples (92% positive). Using the alkaline-lysis method, DNA was amplified reliably on boiled meat samples (83% positive). Lysis-buffer-only method did not show consistent PCR amplification on the samples used (50% positive). The conclusion was that conventional PCI method and DNA isolation kit showed high reliability in PCR amplification of bovine and porcine meats, both raw and boiled. While high DNA yield was obtained using the alkaline-lysis method, PCR amplification was only successful on boiled samples. Lysis-buffer only method yielded in poor DNA quality and was not able to result in reliable DNA amplificationen_US
dc.language.isoenen_US
dc.publisherUniversitas Brawijayaen_US
dc.relation.ispartofseriesThe Journal of Experimental Life Science;Vol.7, Issue 2, Page 110-114
dc.subjectDNA extraction methodsen_US
dc.subjectHalalen_US
dc.subjectPorcine food contaminants.en_US
dc.titleComparison of DNA Extraction Methods Between Conventional, Kit, Alkali and Buffer-Only for PCR Amplification on Raw and Boiled Bovine and Porcine Meaten_US
dc.typeArticleen_US


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