In this case the objective of the research is to know what are the factors that caused students’ anxiety in reading comprehension. This research was conducted in Islamic school at junior high school Miftahul Ulum Sumber kembang Pamekasan. Qualitative design was applied in this research the participants of this research are the students ninth grade of junior high school miftahul ulum sumber kembang pamekasan. The instrument used were questionnaires, observation and interview. Questionnaires used were Foreign language Reading Anxiety Scale (FLRAS) was adopted from Saito et al. (1999). The observation researcher uses a nonparticipant observation and interview used semi-structured interview to obtain depth information related to issues. The findings of this research are students’ anxiety in reading comprehension is caused of the first teacher's teaching method. The participants pointed out that they felt anxious when the teacher directly asked them to read in front of all their friends without teaching them first. Second Fear of negative evaluation because the teacher likes to give bad feedback and angry when the students make a mistake. The last is lack of English proficiency because the students do not know about English lesson due to their lack of vocabulary mastery. The fact, it is suggested that students feel anxiety in reading comprehension because of teachers’ teaching method, fear of negative evaluation, and lack of English proficiency