Molecular Docking of Soybean (Glycine max) Seed and Ginger (Zingiber officinale) Rhizome Components as Anti-Diabetic Through Inhibition of Dipeptidyl Peptidase 4 (DPP-4) and Alpha-Glucosidase Enzymes
JavaScript is disabled for your browser. Some features of this site may not work without it.
Dalam rangka peningkatan layanan dan perbaikan sistem, mohon maaf untuk sementara waktu Repositori UNISMA tidak dapat diakses secara optimal.
Molecular Docking of Soybean (Glycine max) Seed and Ginger (Zingiber officinale) Rhizome Components as Anti-Diabetic Through Inhibition of Dipeptidyl Peptidase 4 (DPP-4) and Alpha-Glucosidase Enzymes
Purnomo, Yudi; Taufiq, Muhammad; Wijaya, Afnan N. D.; Hakim, Reza
Dipeptidyl Peptidase-4 (DPP-4) and alpha-glucosidase (α-glucosidase) are enzymes involved in carbohydrate metabolism. Inhibition of these enzymes contribute to blood glucose level suppression. Soybean (Glycine max) seeds and ginger (Zingiber officinale) rhizome are herbs that have anti-diabetic activity. The mechanism of action, however, has not been thoroughly explored. This study aims to evaluate the anti-diabetic potentials of the chemical components in soybean seeds and ginger rhizome through inhibition activity of DPP-4 and α-glucosidase in silico. Soybean seed and ginger rhizome were extracted using the maceration method with ethanol solvent. Ethanol extract of soybean seeds and ginger rhizome were analysed using Liquid Chromatography-Mass Spectrometry (LC-MS/MS). The potency of active compounds from the plants on DPP-4 and α-glucosidase were evaluated by in silico study using web-based software (Docking server). Soybean seed were found to contain phytosterols, mainly beta sitosterol, campesterol, stigmasterol, and lanosterol. Meanwhile, ginger rhizome was found to contain 6-gingerdiol, 10-gingerol and 12-shogaol. Molecular docking study showed that stigmasterol and 12-shogaol strongly inhibits DPP-4 activity while stigmasterol and 6-Gingerdiol strongly inhibited α-glucosidase. This shows that both soybean seed and ginger rhizome potentially act as an anti-diabetic by inhibiting DPP-4 and α-glucosidase; however, soybean seed is more potent due to its ability to inhibit both of the tested enzymes.
Description:
[ARCHIVES] Copyright Article From:Tropical Journal of Natural Product Research (TJNPR)