Effect of ascorbic acid, α-tocopherol and its combination on human umbilical vein endothelial cells permeability exposed to high concentration of glucose

Purnomo, Yudi; Soeatmadji, Djoko W; Widodo, M Aris

dc.contributor.author	Purnomo, Yudi
dc.contributor.author	Soeatmadji, Djoko W
dc.contributor.author	Widodo, M Aris
dc.date.accessioned	2021-10-11T06:58:09Z
dc.date.available	2021-10-11T06:58:09Z
dc.date.issued	2019-05-28
dc.identifier.issn	2304-3415
dc.identifier.uri	https://romj.org/2020-0201
dc.identifier.uri	http://repository.unisma.ac.id/handle/123456789/1929
dc.description	[ARCHIVES] Copyright Article from: Russian Open Medical Journal	en_US
dc.description.abstract	Aim — To evaluate the effect of ascorbic acid, α-tocopherol, and its combination on the permeability of human umbilical vein endothelial cells (HUVEC’S) culture after high glucose concentration exposure. Material and Methods — The study was performed using HUVEC’S culture on transwell polyester filter. The ascorbic acid, α-tocopherol, and its combination (25-200 µM) were concomitantly given with glucose 22 µM to the culture media. After seven days of treatment, endothelial permeability was observed by measuring the concentration of trypan blue-labeled albumin in a luminal compartment with a spectrophotometer (λ=592 nm). The morphology of the cells was observed under an inverted microscope. The data was analyzed using ANOVA test followed by Dunnet test (p<0.05). Results — HUVEC’S culture exposed to high concentration glucose increased endothelial permeability better than those which exposed to normal concentration (p<0.05). The treatment of ascorbic acid 200 µM, α-tocopherol 100 µM and 200 µM were capable to inhibit the increase HUVEC’S permeability exposed to high concentration glucose better than control group (p<0.05). Moreover, two combinations of ascorbic acid and α-tocopherol, respectively 50 and 100 µM, could prevent the increased permeability of HUVEC’S culture better than control (p<0.05). Conclusion — Ascorbic acid, α-tocopherol, and its combination could inhibit the increase of endothelial permeability exposed to a high concentration of glucose.	en_US
dc.language.iso	en	en_US
dc.publisher	Russian Open Medical Journal	en_US
dc.relation.ispartofseries	Russian Open Medical Journal;Vol.9, Issue 2, Pages 1-5
dc.subject	ascorbic acid	en_US
dc.subject	α-tocopherol	en_US
dc.subject	permeability	en_US
dc.subject	endothelial cells	en_US
dc.subject	high glucose	en_US
dc.title	Effect of ascorbic acid, α-tocopherol and its combination on human umbilical vein endothelial cells permeability exposed to high concentration of glucose	en_US
dc.type	Article	en_US