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dc.contributor.authorPurnomo, Yudi
dc.contributor.authorSoeatmadji, Djoko W
dc.contributor.authorWidodo, M Aris
dc.date.accessioned2021-10-11T06:58:09Z
dc.date.available2021-10-11T06:58:09Z
dc.date.issued2019-05-28
dc.identifier.issn2304-3415
dc.identifier.urihttps://romj.org/2020-0201
dc.identifier.urihttp://repository.unisma.ac.id/handle/123456789/1929
dc.description[ARCHIVES] Copyright Article from: Russian Open Medical Journalen_US
dc.description.abstractAim — To evaluate the effect of ascorbic acid, α-tocopherol, and its combination on the permeability of human umbilical vein endothelial cells (HUVEC’S) culture after high glucose concentration exposure. Material and Methods — The study was performed using HUVEC’S culture on transwell polyester filter. The ascorbic acid, α-tocopherol, and its combination (25-200 µM) were concomitantly given with glucose 22 µM to the culture media. After seven days of treatment, endothelial permeability was observed by measuring the concentration of trypan blue-labeled albumin in a luminal compartment with a spectrophotometer (λ=592 nm). The morphology of the cells was observed under an inverted microscope. The data was analyzed using ANOVA test followed by Dunnet test (p<0.05). Results — HUVEC’S culture exposed to high concentration glucose increased endothelial permeability better than those which exposed to normal concentration (p<0.05). The treatment of ascorbic acid 200 µM, α-tocopherol 100 µM and 200 µM were capable to inhibit the increase HUVEC’S permeability exposed to high concentration glucose better than control group (p<0.05). Moreover, two combinations of ascorbic acid and α-tocopherol, respectively 50 and 100 µM, could prevent the increased permeability of HUVEC’S culture better than control (p<0.05). Conclusion — Ascorbic acid, α-tocopherol, and its combination could inhibit the increase of endothelial permeability exposed to a high concentration of glucose.en_US
dc.language.isoenen_US
dc.publisherRussian Open Medical Journalen_US
dc.relation.ispartofseriesRussian Open Medical Journal;Vol.9, Issue 2, Pages 1-5
dc.subjectascorbic aciden_US
dc.subjectα-tocopherolen_US
dc.subjectpermeabilityen_US
dc.subjectendothelial cellsen_US
dc.subjecthigh glucoseen_US
dc.titleEffect of ascorbic acid, α-tocopherol and its combination on human umbilical vein endothelial cells permeability exposed to high concentration of glucoseen_US
dc.typeArticleen_US


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