Abstract:
Aim — To evaluate the effect of ascorbic acid, α-tocopherol, and its combination on the permeability of human umbilical vein
endothelial cells (HUVEC’S) culture after high glucose concentration exposure.
Material and Methods — The study was performed using HUVEC’S culture on transwell polyester filter. The ascorbic acid, α-tocopherol,
and its combination (25-200 µM) were concomitantly given with glucose 22 µM to the culture media. After seven days of treatment,
endothelial permeability was observed by measuring the concentration of trypan blue-labeled albumin in a luminal compartment with a
spectrophotometer (λ=592 nm). The morphology of the cells was observed under an inverted microscope. The data was analyzed using
ANOVA test followed by Dunnet test (p<0.05).
Results — HUVEC’S culture exposed to high concentration glucose increased endothelial permeability better than those which exposed to
normal concentration (p<0.05). The treatment of ascorbic acid 200 µM, α-tocopherol 100 µM and 200 µM were capable to inhibit the
increase HUVEC’S permeability exposed to high concentration glucose better than control group (p<0.05). Moreover, two combinations of
ascorbic acid and α-tocopherol, respectively 50 and 100 µM, could prevent the increased permeability of HUVEC’S culture better than
control (p<0.05).
Conclusion — Ascorbic acid, α-tocopherol, and its combination could inhibit the increase of endothelial permeability exposed to a high
concentration of glucose.
