The purpose of the study was to give public scientific information on the use of lime juice to enhance physical quality and the saving power of pureblood chicken at room temperature, especially on the number of bacteria, WHC and cowhizing of meat. The materials used in research are 35 days of ras chicken, lime (Citrus aurantifolia) age 28 weeks for as much as 1 kg (2 lb), aquades, Nutrient Agar (NA), and 70% alcohol, polyethylene (PE) plastic, wathman 42 diameter 11 cm paper, graph paper. The method in this study uses a completely randomized design (CRD) consisting of 12 combination treatments and 3 replications. The treatments used in this study were a combination of immersion length (P = 0, Q = 3, Q = 6, and R = 9 hours) and concentration (A = 10, B = 15, and C = 20%). Variance analysis showed that the interaction of storage time at room temperature and concentration of lime solution has a very significant effect (P <0.01) on the number of bacteria and the WHC (Water Holding Capacity) but have xi no visible effect on cooking losses. The result of the transformational bacteria calculation were CP = 5.43 ± 0.04; CQ = 5.54 ± 0.09; CR = 5.73 ± 0.10; CS = 5.99c ± 0.02; BP = 5.79 ± 0.02; BQ = 5.87 ± 0.02; BR = 5.92 ± 0.01; BS = 5.99 ± 0.01; AP = 6.10 ± 0.02; AQ = 6.20 ± 0.01; AR = 6.34 ± 0.04; AS = 6.45 ± 0.02. The results of the WHC calculation (%) CS = 5; CR = 6; CQ = 7; BS = 8; CP = 9; BR = 10; BQ = 11; BP = 13; AS = 14; AR = 18; AQ = 22; AP = 25, and for the results of cooking losses (%) is AP = 46; AQ = 48; AR = 48; AS = 50; BP = 50; BQ = 51; BR = 54; BS = 55; CP = 56; CQ = 58; CR = 59; CS = 60. The conclusion of this study is that the higher the concentration of lime solution will be able to reduce the number of bacteria and WHC but increase the value of cooking losses, the longer the storage of broiler chicken will further increase the number of bacteria and decrease the WHC value and increase the value of cooking losses. It is recommended that before storing broiler chicken at room temperature it should be soaked in lime juice first and it is recommended not to store broiler chicken for more than 6 hours at room temperature even though it has been immersed in a solution of lime. Keywords: meat storage duration, concentration of lemon juice, microbial count, WHC, cooking losses.